2F2E
Crystal Structure of PA1607, a Putative Transcription Factor
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Collection date | 2005-04-01 |
| Wavelength(s) | 0.97932, 0.97948 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 46.884, 78.865, 78.941 |
| Unit cell angles | 90.00, 91.64, 90.00 |
Refinement procedure
| Resolution | 35.270 - 1.850 |
| R-factor | 0.18081 |
| Rwork | 0.177 |
| R-free | 0.25099 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.696 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 78.000 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 24599 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 298 | 10mg/ml protein, 0.1 M Tris, 0.2 M LiSO4, 28 % PEG 3350, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
