2F0R
Crystallographic structure of human Tsg101 UEV domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-05 |
| Detector | BRUKER PROTEUM X8 |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 |
| Unit cell lengths | 97.865, 97.865, 110.583 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.260 |
| R-factor | 0.19059 |
| Rwork | 0.188 |
| R-free | 0.24271 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s1q |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.785 |
| Data reduction software | SAINT |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.314 |
| High resolution limit [Å] | 2.250 | 2.255 |
| Rmerge | 0.030 | |
| Number of reflections | 17160 | |
| <I/σ(I)> | 26.3 | |
| Completeness [%] | 92.4 | 91.73 |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 288 | 20% PEG 4000, 0.2 M ammonium sulphate, 0.1 M Tris , pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
