2F03
Crystal structure of tetrameric restriction endonuclease SfiI in complex with cognate DNA (partial bound form)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Wavelength(s) | 0.928, 0.97931 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 85.600, 85.600, 418.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.050 |
| Rwork | 0.241 |
| R-free | 0.29300 |
| Structure solution method | MAD |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.050 |
| Number of reflections | 31321 |
| Completeness [%] | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | pH 4.6-5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
