2EZ1

Holo tyrosine phenol-lyase from Citrobacter freundii at pH 8.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1995-06-22
Detector	MARRESEARCH
Wavelength(s)	0.87
Spacegroup name	P 21 21 2
Unit cell lengths	133.860, 143.850, 60.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.840 - 1.900
R-factor	0.153
Rwork	0.152
R-free	0.18600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.312
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.075	0.359
Number of reflections	85710
Completeness [%]	92.0	90.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOUR DIFFUSION, HANGING DROP	8	293	50 mM triethanolamine buffer, 0.5 mM PLP, 2 mM DDT, 0.4 M to 0.8 M KCl, 35 - 38% (w/v) monomethyl ether PEG 5000, pH 8.0, VAPOUR DIFFUSION, HANGING DROP, temperature 293K