2EZ0
Crystal structure of the S107A/E148Q/Y445A mutant of EcClC, in complex with a FaB fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-14 |
Detector | MARRESEARCH |
Wavelength(s) | 0.91931 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 220.155, 124.032, 149.282 |
Unit cell angles | 90.00, 127.97, 90.00 |
Refinement procedure
Resolution | 19.920 - 3.540 |
R-factor | 0.258 |
Rwork | 0.258 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1otu |
RMSD bond length | 0.015 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.620 |
High resolution limit [Å] | 3.500 | 3.500 |
Number of reflections | 33492 | |
Completeness [%] | 89.1 | 90.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 36% peg200/300 1:2, 100mM NaBr, 50 mM NaKTart, 50 mM Glycine, pH 9.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |