2EXK
Structure of the family43 beta-Xylosidase E187G from geobacillus stearothermophilus in complex with xylobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.8048 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 139.870, 139.870, 232.025 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 2.230 - 2.200 |
R-factor | 0.188 |
Rwork | 0.185 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.340 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.230 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 116620 | |
Completeness [%] | 99.5 | 97.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.4 | 285 | 17% PEG 6000, 0.1M MES, pH 5.4, VAPOR DIFFUSION, SITTING DROP, temperature 285K |