2EWW

Crystal Structure of the Pilus Retraction Motor PilT and Bound ATP

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-01
Detector	MARRESEARCH
Wavelength(s)	0.9794
Spacegroup name	P 6
Unit cell lengths	107.380, 107.380, 68.860
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.470 - 3.200
R-factor	0.24236
Rwork	0.239
R-free	0.30981
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.149
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.079	0.256
Number of reflections	7481
<I/σ(I)>	7.9	2.9
Completeness [%]	99.5	98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	293	5-8 mg/ml protein 30-45% MPD 0.2-0.4 M Ammonium Sulfate Tris, 15mM KCl 75mM 5% glycerol 5-10mM magnesium Chloride 1-5 mM ATP , pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.9	293	5-8 mg/ml protein 30-45% MPD 0.2-0.4 M Ammonium Sulfate Tris, 15mM KCl 75mM 5% glycerol 5-10mM magnesium Chloride 1-5 mM ATP , pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K