2EWO
X-ray structure of putative agmatine deiminase Q8DW17, Northeast Structural Genomics target SmR6.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-25 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97938 0.97952 0.96793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.524, 203.685, 139.543 |
| Unit cell angles | 90.00, 104.72, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.900 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.26900 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.145 | 0.696 |
| Number of reflections | 218714 | |
| <I/σ(I)> | 9.1 | 1.99 |
| Completeness [%] | 99.8 | |
| Redundancy | 4.2 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.1 | 294 | 16% PEG6K, 100mM Tris-HCl, 400mM LiCl, pH 9.1, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
