2EWJ
Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- Locked form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2005-10-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.679, 62.679, 251.619 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.22259 |
| Rwork | 0.219 |
| R-free | 0.30260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ecr |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.067 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.112 | 0.529 |
| Number of reflections | 14359 | |
| <I/σ(I)> | 9.3 | 1.9 |
| Completeness [%] | 97.9 | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 291 | 13% PEG 3350, 0.2M sodium iodide, 50mM Bis-Tris pH 6.75, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | sodium iodide | ||
| 3 | 1 | 1 | water | ||
| 4 | 1 | 2 | PEG 3350 | ||
| 5 | 1 | 2 | sodium iodide |
