2EWG
T. brucei Farnesyl Diphosphate Synthase Complexed with Minodronate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 123.2 |
| Detector technology | CCD |
| Collection date | 2003-08-01 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 131.981, 118.098, 63.246 |
| Unit cell angles | 90.00, 112.48, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.480 |
| R-factor | 0.242 |
| R-free | 0.25980 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.018 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.590 |
| High resolution limit [Å] | 2.480 | 2.500 |
| Number of reflections | 30546 | |
| Completeness [%] | 96.5 | 79.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.75 | 277.15 | 20% Ethylene Glycol, 0.1 Ammonium Acetate, pH 5.75, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
