2EVV
Crystal Structure of the PEBP-like Protein of Unknown Function HP0218 from Helicobacter pylori
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-04-03 |
Detector | SBC-3 |
Wavelength(s) | 0.97922 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 102.312, 102.266, 143.047 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.750 - 2.590 |
R-factor | 0.2235 |
Rwork | 0.219 |
R-free | 0.26248 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.441 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.750 | 2.680 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.191 | 0.798 |
Number of reflections | 47122 | |
<I/σ(I)> | 4.9 | 2.2 |
Completeness [%] | 97.6 | 79.7 |
Redundancy | 11.1 | 8.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 0.1M Bis-Tris PH6.0, 0.2M Ammonium Sulphate, 20%PEG4K, 2% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |