2EV5
Bacillus subtilis manganese transport regulator (MNTR) bound to calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0781 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.690, 45.770, 74.730 |
| Unit cell angles | 90.00, 93.76, 90.00 |
Refinement procedure
| Resolution | 39.000 - 2.000 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1on1 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.130 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.109 | 0.278 |
| Number of reflections | 21041 | |
| <I/σ(I)> | 4.4 | |
| Completeness [%] | 91.8 | 91.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1M Tris HCl, 0.2M calcium acetate, 18% PEG 8000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
