2EU1
Crystal structure of the chaperonin GroEL-E461K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 267.695, 290.641, 247.006 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.290 |
| R-factor | 0.276 |
| Rwork | 0.276 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oel |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.329 |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.750 |
| High resolution limit [Å] | 3.290 |
| Number of reflections | 123350 |
| Completeness [%] | 85.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 277 | Reservoir solution: 43% (v/v) MPD, 100mM Imidazole, 170mM MgCl2.6H2O, dissolved in the ratio 1:1.5 with 22.5mg/ml protein, 50mM TRIS-HCL, 10mM MgCl2, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
