2ETN

Crystal structure of Thermus aquaticus Gfh1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	77
Detector technology	CCD
Collection date	2003-06-30
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	191.200, 76.400, 53.100
Unit cell angles	90.00, 101.90, 90.00

Refinement procedure

Resolution	30.000 - 3.300
R-factor	0.25742
Rwork	0.250
R-free	0.32464
Structure solution method	MAD
RMSD bond length	0.033
RMSD bond angle	3.009
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.310
High resolution limit [Å]	3.200	3.200
Number of reflections	12359
<I/σ(I)>	18.5	2.2
Completeness [%]	99.2	99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	50mM sodium Cacodylate pH 6.0, 10 mM magnesium sulfate, 1.9 M lithium sulfate 1% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	6	295	50mM sodium Cacodylate pH 6.0, 10 mM magnesium sulfate, 1.9 M lithium sulfate 1% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 295K