2ETJ
Crystal structure of Ribonuclease HII (EC 3.1.26.4) (RNase HII) (tm0915) from THERMOTOGA MARITIMA at 1.74 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-29 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9797, 1.0000 |
Spacegroup name | P 31 |
Unit cell lengths | 51.821, 51.821, 76.293 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.150 - 1.740 |
Rwork | 0.179 |
R-free | 0.21900 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.572 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 18.150 | 18.150 | 1.790 |
High resolution limit [Å] | 1.740 | 7.780 | 1.740 |
Rmerge | 0.069 | 0.048 | 0.469 |
Number of reflections | 23391 | ||
<I/σ(I)> | 7.1 | 14 | 1.5 |
Completeness [%] | 99.4 | ||
Redundancy | 2.8 | 2.5 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 5.8 | 277 | 0.2M MgCl2, 20.0% PEG-3350, No Buffer , pH 5.8, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |