2ETC
Crystal structure of the ankyrin repeat domain of TRPV2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-04 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.514, 95.862, 164.504 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.21499 |
| Rwork | 0.210 |
| R-free | 0.26184 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eta |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.961 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.140 | 0.493 |
| Number of reflections | 11944 | |
| <I/σ(I)> | 17.4 | 5.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 0.33mM protein, 9% PEG8000, 0.1M sodium citrate, pH 5.0, 5-7% xylitol, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
