2ERG
Crystal Structure of Leu3 DNA-binding domain with a single H50C mutation complexed with a 15mer DNA duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2000-06-08 |
| Wavelength(s) | 0.9213 |
| Spacegroup name | P 62 |
| Unit cell lengths | 66.768, 66.768, 122.745 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.090 - 3.150 |
| R-factor | 0.273 |
| Rwork | 0.273 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ere |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 5419 | |
| <I/σ(I)> | 3.7 | 2.9 |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 50 mM Mes, 3% v/v isopropanol, 20 mM Mg(OAc)2, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Mes | ||
| 2 | 1 | 1 | isopropanol | ||
| 3 | 1 | 1 | Mg(OAc)2 | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Mes | ||
| 6 | 1 | 2 | Mg(OAc)2 | ||
| 7 | 1 | 2 | H2O |
