2EPI
Crystal Structure pf hypothetical protein MJ1052 from Methanocaldococcus jannascii (Form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-09 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.780, 75.747, 54.991 |
| Unit cell angles | 90.00, 117.28, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lxn |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.061 | 0.376 |
| Number of reflections | 41290 | |
| <I/σ(I)> | 11.9 | 2.24 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.9 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7 | 295 | 30%(v/v) Jeffamine M-600, 0.1M HEPES, pH 7.0, microbatch, temperature 295K |
