2EP5
Structural study of Project ID ST1242 from Sulfolobus tokodaii strain7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-06 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.157, 73.986, 91.552 |
| Unit cell angles | 68.10, 68.39, 86.04 |
Refinement procedure
| Resolution | 29.270 - 2.400 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.063 | 0.193 |
| Number of reflections | 58141 | |
| <I/σ(I)> | 11.4 | 4.18 |
| Completeness [%] | 94.8 | 94.4 |
| Redundancy | 2.3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 291 | 8w/v(%) PEG 4K, 0.1M Acet, pH 4.6, MICROBATCH, temperature 291K |
