2EO0
Crystal Structure of Holliday Junction Resolvase ST1444
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.661, 49.326, 69.894 |
| Unit cell angles | 90.00, 103.28, 90.00 |
Refinement procedure
| Resolution | 25.870 - 2.400 |
| R-factor | 0.2325 |
| Rwork | 0.232 |
| R-free | 0.31200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hh1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.485 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.064 | 0.508 |
| Number of reflections | 11787 | |
| <I/σ(I)> | 14.4 | 3.75 |
| Completeness [%] | 99.6 | 98 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M HEPES, 1.4M tri-Sodium Citrate dehydrate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
