2ENX
Structure of the family II inorganic pyrophosphatase from Streptococcus agalactiae at 2.8 resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-06 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 181.960, 181.960, 132.610 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.500 - 2.800 |
| R-factor | 0.19565 |
| Rwork | 0.192 |
| R-free | 0.26695 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i74 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.513 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.140 | 0.300 |
| Number of reflections | 20700 | |
| <I/σ(I)> | 12.3 | 6.2 |
| Completeness [%] | 99.6 | 96.2 |
| Redundancy | 4.2 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.2 | 298 | 0.4M ammonium phosphate monobasic, pH 4.2, VAPOR DIFFUSION, temperature 298K |
