2EMQ
Hypothetical Conserved Protein (GK1048) from Geobacillus Kaustophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-16 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 65 |
| Unit cell lengths | 56.194, 56.194, 211.224 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.231 |
| Rwork | 0.231 |
| R-free | 0.29300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yav |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.322 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.088 | 0.526 |
| Number of reflections | 12961 | |
| <I/σ(I)> | 18 | 12.77 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 16.4 | 16.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2M Li2SO4, 0.1 M Tris, 1M Na tartrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
