2ELG
The rare crystallographic structure of d(CGCGCG)2: The natural spermidine molecule bound to the minor groove of left-handed Z-DNA d(CGCGCG)2 at 10 degree celsius
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2005-12-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 17.847, 30.988, 44.022 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 7.000 - 1.000 |
| R-factor | 0.244 |
| Rwork | 0.232 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dj6 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MERLOT |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.047 | 0.047 |
| Number of reflections | 11287 | |
| <I/σ(I)> | 20.7 | 20.7 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 4.7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 283 | 10mM Sodium cacodilate, 1M Magnesium chloride, 100mM spermidine trichloride, 8%MPD, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Sodium cacodilate | ||
| 2 | 1 | 1 | Magnesium chloride | ||
| 3 | 1 | 1 | spermidine trichloride | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 1 | HOH | ||
| 6 | 1 | 2 | Sodium cacodilate | ||
| 7 | 1 | 2 | Magnesium chloride | ||
| 8 | 1 | 2 | MPD | ||
| 9 | 1 | 2 | HOH |
