2EL9
Crystal structure of E.coli Histidyl-tRNA synthetase complexed with a histidyl-adenylate analogue
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-07-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.113, 108.345, 193.645 |
Unit cell angles | 90.00, 98.21, 90.00 |
Refinement procedure
Resolution | 48.800 - 2.700 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ady |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 64552 | |
Completeness [%] | 95.4 | 85.1 |
Redundancy | 6.4 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES, 0.5M NaCl, 14% PEG 4000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |