2EKD
Structural study of Project ID PH0250 from Pyrococcus horikoshii OT3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-04-19 |
Detector | RIGAKU RAXIS V |
Wavelength(s) | 0.97907 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 93.471, 158.026, 163.347 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.940 - 2.300 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.22500 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.081 | 0.148 |
Number of reflections | 54164 | |
<I/σ(I)> | 20.8 | 12.16 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.3 | 291 | 40%(v/v) 1,2-propanediol, Acetate pH 4.5, 0.05M Ca(OAc)2, pH 5.3, MICROBATCH, temperature 291K |