2EJN
Structural characterization of the tetrameric form of the major cat allergen fel D 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-02 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.087 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.588, 42.996, 48.906 |
| Unit cell angles | 70.80, 80.56, 81.53 |
Refinement procedure
| Resolution | 36.510 - 1.640 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1puo |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.510 |
| High resolution limit [Å] | 1.640 |
| Number of reflections | 31695 |
| Completeness [%] | 92.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 22.5% PEG3350, 0.5M CaCl2, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
