2EIF

Eukaryotic translation initiation factor 5A from Methanococcus jannaschii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-07-01
Detector	RIGAKU RAXIS IIC
Spacegroup name	C 1 2 1
Unit cell lengths	80.483, 40.198, 48.580
Unit cell angles	90.00, 124.29, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.214
Rwork	0.214
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eif
RMSD bond length	0.011
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.045	0.233
Number of reflections	11659
<I/σ(I)>	16.2	3.7
Completeness [%]	96.7	91.2
Redundancy	1.98	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.5	4 *	0.1M TRIS-HCL (PH 8.5), 0.2M MGCL2, 30% PEG4000
1	Vapor diffusion *	8.5	4 *	0.1M TRIS-HCL (PH 8.5), 0.2M MGCL2, 30% PEG4000
1	Vapor diffusion *	8.5	4 *	0.1M TRIS-HCL (PH 8.5), 0.2M MGCL2, 30% PEG4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	0.1 (M)
3	1	drop		0.2 (M)
4	1	drop	PEG4000	30 (%)