2EGY
Crystal structure of LysN, alpha-aminoadipate aminotransferase (substrate free form), from Thermus thermophilus HB27
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-17 |
| Detector | ADSC QUAMTUM 4r |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.415, 138.510, 111.745 |
| Unit cell angles | 90.00, 100.21, 90.00 |
Refinement procedure
| Resolution | 43.970 - 2.670 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2DTV |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.670 |
| High resolution limit [Å] | 2.670 | 2.670 |
| Rmerge | 0.062 | 0.232 |
| Number of reflections | 48044 | |
| <I/σ(I)> | 4.3 | |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | 17% PEG 4000, 0.1M tri-Sodium Citrate (pH6.6), 0.2M Ammonium Sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
