2EGW
Crystal structure of rRNA methyltransferase with SAH ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2006-10-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 112.637, 38.774, 115.286 |
Unit cell angles | 90.00, 115.73, 90.00 |
Refinement procedure
Resolution | 39.350 - 2.800 |
R-factor | 0.226 |
Rwork | 0.226 |
R-free | 0.30700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 11338 | |
<I/σ(I)> | 23.9231 | 7.34286 |
Completeness [%] | 99.9 | 99.5 |
Redundancy | 3.84927 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | VAPOR DIFFUSION, SITTING DROP |