2EGU
Crystal structure of O-acetylserine sulfhydrase from Geobacillus kaustophilus HTA426
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-16 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.250, 72.250, 127.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.810 - 1.900 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1Z7W.pdb |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.353 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.107 | 0.529 |
| Number of reflections | 27236 | |
| <I/σ(I)> | 36.86 | 5.22 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.3 | 14.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 293 | 100mM MES-NaOH, 50mM MgCl2, 27.5% PEG 4000, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
