2EGK

Crystal Structure of Tamalin PDZ-Intrinsic Ligand Fusion Protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	298
Detector technology	CCD
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.97910, 0.97940, 0.99000
Spacegroup name	P 21 21 21
Unit cell lengths	48.560, 114.070, 125.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.850
Rwork	0.269
R-free	0.29060
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.850	2.850
Rmerge	0.108	0.396
Number of reflections	29994
<I/σ(I)>	5.9
Completeness [%]	97.2
Redundancy	3.7	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1M HEPES-Na, 0.8M sodium dihydrogen phosphate, 0.8M potassium dihydrogen phosphate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K