2EFY

Crystal Structure of T.th. HB8 O-acetylserine sulfhydrylase Complexed with 4-Acetylbutyric acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-14
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 43
Unit cell lengths	100.760, 100.760, 65.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.110 - 2.350
R-factor	0.193
Rwork	0.193
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ve1
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	26941
Completeness [%]	98.2	99.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	293	tri-sodium citrate, CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K