2EFR
Crystal structure of the c-terminal tropomyosin fragment with N- and C-terminal extensions of the leucine zipper at 1.8 angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-06-07 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.363, 65.840, 71.135 |
| Unit cell angles | 66.40, 89.93, 90.03 |
Refinement procedure
| Resolution | 19.800 - 1.800 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.31600 |
| Structure solution method | MR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.064 | 0.201 |
| Number of reflections | 62779 | |
| <I/σ(I)> | 17.1 | 5.5 |
| Completeness [%] | 96.3 | 95.1 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 2.2M 1,6-HEXANEDIOL, 0.2M AMMONIUM ACETATE, 0.1M HEPES, PH7.5, pH 7.50, VAPOR DIFFUSION, SITTING DROP |
