2EFN
Crystal Structure of Ser 32 to Ala of ST1022 from Sulfolobus tokodaii 7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2006-12-15 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.9793 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 103.662, 103.662, 73.146 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.950 - 1.980 |
R-factor | 0.209 |
Rwork | 0.209 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2e7w |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.050 |
High resolution limit [Å] | 1.980 | 1.980 |
Rmerge | 0.099 | 0.465 |
Number of reflections | 14388 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 14.7 | 14.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 20%iso PolypropyleneGlycol, 30% isopropanol 0.07M Sodium citrate, 10mM FeCl3-additive, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |