2EFD
Ara7/AtVps9a
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.91980 |
| Spacegroup name | P 61 |
| Unit cell lengths | 189.544, 189.544, 75.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.950 - 3.000 |
| R-factor | 0.30797 |
| Rwork | 0.306 |
| R-free | 0.35126 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.091 | 0.633 |
| Number of reflections | 29805 | |
| <I/σ(I)> | 27.6 | 3.5 |
| Completeness [%] | 96.1 | 100 |
| Redundancy | 11.2 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 3% PEG4000, 50mM imidazole malate, 50mM NaCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 3% PEG4000, 50mM imidazole malate, 50mM NaCl, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
