2EF9
Structural and mechanistic changes along an engineered path from metallo to non-metallo KDO8P synthase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Wavelength(s) | 1.5418 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 84.574, 84.574, 159.742 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.160 - 2.000 |
R-factor | 0.188 |
Rwork | 0.188 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fwn |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 26.160 |
High resolution limit [Å] | 2.000 |
Number of reflections | 44624 |
Completeness [%] | 1.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |