2EF7
Crystal structure of ST2348, a hypothetical protein with CBS domains from Sulfolobus tokodaii strain7
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-17 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97897, 0.9, 0.97929 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 51.420, 135.272, 41.234 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.440 - 2.100 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.26000 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.063 | 0.251 |
| Number of reflections | 17492 | |
| <I/σ(I)> | 32.5 | 3.89 |
| Completeness [%] | 99.6 | 97.3 |
| Redundancy | 6.7 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 293 | 160mM ammonium chloride, 16%(w/v) polyethylene glycol 3350, pH 6.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
