2EF6
Canavalia gladiata lectin complexed with Man1-3Man-OMe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-01 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1.43 |
| Spacegroup name | P 32 |
| Unit cell lengths | 69.391, 69.391, 161.298 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.920 - 2.100 |
| R-factor | 0.19224 |
| Rwork | 0.189 |
| R-free | 0.24526 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wuv |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.267 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.290 | |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.040 | 0.452 |
| Number of reflections | 48437 | |
| <I/σ(I)> | 10.7 | 2 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | Sodium Formate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
