2EES
Guanine riboswitch A21U, U75A mutant bound to hypoxanthine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-22 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.540, 35.960, 41.470 |
| Unit cell angles | 90.00, 91.31, 90.00 |
Refinement procedure
| Resolution | 19.910 - 1.750 |
| Rwork | 0.207 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1U8D |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.100 |
| Data reduction software | d*TREK |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.810 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 19129 | |
| <I/σ(I)> | 15.9 | 4.1 |
| Completeness [%] | 98.3 | 85.2 |
| Redundancy | 3.42 | 2.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20% Polyethylene glycol 3000, 640mM ammonium acetate, 10mM cobalt hexammine chloride, 10mM K(+)-HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Polyethylene glycol 3000 | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | ammonium acetate | ||
| 3 | 1 | 1 | cobalt hexammine chloride | ||
| 4 | 1 | 1 | K(+)-HEPES | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | Polyethylene glycol 3000 | ||
| 7 | 1 | 2 | ammonium acetate | ||
| 8 | 1 | 2 | cobalt hexammine chloride | ||
| 9 | 1 | 2 | K(+)-HEPES |
