2ECS
Lambda Cro mutant Q27P/A29S/K32Q at 1.4 A in space group C2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-19 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.689, 26.999, 55.523 |
| Unit cell angles | 90.00, 103.69, 90.00 |
Refinement procedure
| Resolution | 53.920 - 1.400 |
| R-factor | 0.141 |
| Rwork | 0.139 |
| R-free | 0.17500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Lambda Cro Q27P/A29S/K32Q at 1.35 A resolution in space group P3221 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.505 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.946 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.074 | 0.416 |
| Total number of observations | 19965 | |
| Number of reflections | 25562 | |
| <I/σ(I)> | 5.8 | 1.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.5 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 80% saturated lithium sulfate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
