2EC2
Crystal structure of transposase from Sulfolobus tokodaii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-03-10 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | P 1 |
Unit cell lengths | 55.118, 66.566, 69.472 |
Unit cell angles | 107.96, 104.39, 110.72 |
Refinement procedure
Resolution | 25.780 - 2.800 |
R-factor | 0.20629 |
Rwork | 0.201 |
R-free | 0.29861 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f5g |
RMSD bond length | 0.011 |
RMSD bond angle | 1.385 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.057 | 0.230 |
Number of reflections | 18474 | |
<I/σ(I)> | 28.1 | |
Completeness [%] | 93.0 | 82 |
Redundancy | 2.9 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 20% PEG 4000, 0.2M Lithium Sulfate, 0.1M Sodium Acetate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 298.0K |