2EBY
Crystal structure of a hypothetical protein from E. Coli
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 180 |
| Detector technology | CCD |
| Collection date | 2006-12-18 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.97895, 0.97943, 0.9000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 89.793, 89.793, 54.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.930 - 2.250 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.24800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.077 | 0.306 |
| Number of reflections | 12218 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.2 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 9.4 | 293 | 1.93M Ammonium sulfate, 0.1M CHES, pH 9.4, LIQUID DIFFUSION, temperature 293K |
