2EBO
CORE STRUCTURE OF GP2 FROM EBOLA VIRUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-12 |
| Detector | ADSC |
| Spacegroup name | P 62 |
| Unit cell lengths | 75.660, 75.660, 67.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.26900 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mof |
| RMSD bond length | 0.009 |
| RMSD bond angle | 19.800 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.5) |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 1.920 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.037 | 0.309 |
| Total number of observations | 86630 * | |
| Number of reflections | 17326 | |
| <I/σ(I)> | 18.8 | 3.5 |
| Completeness [%] | 98.9 | 99.1 |
| Redundancy | 4.9 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | pH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | isopropanol | 16-18 (%) | |
| 3 | 1 | reservoir | sodium acetate | 100 (mM) | pH4.6 |
| 4 | 1 | reservoir | 100-200 (mM) |
