2EB7
Crystal structure of the hypothetical regulator from Sulfolobus tokodaii 7
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2006-07-18 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 0.97896, 0.97930, 1.0000 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 45.919, 45.919, 138.559 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.280 - 1.800 |
R-factor | 0.228 |
Rwork | 0.228 |
R-free | 0.26800 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.000 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.065 | 0.565 |
Number of reflections | 14613 | |
Completeness [%] | 99.4 | 99.7 |
Redundancy | 12.7 | 12.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 18% PEG 8000, 0.1M Sodium cacodylate, 0.2M Calcium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |