2EAY
Crystal Structure Of Biotin Protein Ligase From Aquifex Aeolicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.076, 60.589, 73.106 |
| Unit cell angles | 90.00, 91.99, 90.00 |
Refinement procedure
| Resolution | 36.530 - 1.950 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wqw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.530 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.049 | 0.328 |
| Number of reflections | 33213 | |
| <I/σ(I)> | 14.7 | 2.1 |
| Completeness [%] | 94.2 | 96.4 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.4 | 295 | 27.5w/v(%) PEG 4K, 0.1M Hepes-NaOH, pH 7.4, microbatch, temperature 295K |
