2EAU
Crystal structure of the SR CA2+-ATPASE with bound CPA in the presence of curcumin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.8000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 71.648, 71.648, 586.246 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.2468 |
| Rwork | 0.247 |
| R-free | 0.27180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iwo |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.880 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.141 | 0.534 |
| Number of reflections | 39493 | |
| <I/σ(I)> | 32 | 7.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 17.1 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.1 | 283 | PEG 400, pH 6.1, MICRODIALYSIS, temperature 283K |
