2EAT
Crystal structure of the SR CA2+-ATPASE with bound CPA and TG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.8000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.899, 95.645, 155.100 |
| Unit cell angles | 90.00, 95.24, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| R-factor | 0.2465 |
| Rwork | 0.244 |
| R-free | 0.29223 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iwo |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.416 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.970 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.048 | 0.361 |
| Number of reflections | 40344 | |
| <I/σ(I)> | 33.6 | 2 |
| Completeness [%] | 99.2 | 92.5 |
| Redundancy | 7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.1 | 283 | PEG 400, pH 6.1, MICRODIALYSIS, temperature 283K |
