2E87

Crystal structure of hypothetical GTP-binding protein PH1320 from Pyrococcus horikoshii OT3, in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-04
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.978997
Spacegroup name	P 21 21 21
Unit cell lengths	47.253, 99.191, 126.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.600 - 2.350
R-factor	0.221
Rwork	0.221
R-free	0.25500
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.430
High resolution limit [Å]	2.350	2.350
Number of reflections	25473
<I/σ(I)>	15.8132	2.79832
Completeness [%]	98.5	91.6
Redundancy	4.33557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	300	1.5M ammonium sulfate, 0.2M potassium sodium tartrate tetrahydrate, 0.1M tri-sodium citrate dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300K