2E84
Crystal structure of High-Molecular Weight Cytochrome c from Desulfovibrio vulgaris (Miyazaki F) in the presence of zinc ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-01-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.7000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.090, 84.070, 142.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.200 - 2.700 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.25900 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.053 | 0.115 |
| Number of reflections | 20426 | |
| <I/σ(I)> | 13.3 | 3.6 |
| Completeness [%] | 67.6 | |
| Redundancy | 4.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 294 | PEG 6000, zinc acetate, pH 6.5, VAPOR DIFFUSION, temperature 294K |
