2E4F
Crystal Structure of the Cytoplasmic Domain of G-Protein-Gated Inward Rectifier Potassium Channel Kir3.2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-12-06 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 77.173, 77.173, 87.307 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.302 |
| Rwork | 0.244 |
| R-free | 0.27890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n9p |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.450 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.274 | 2.380 |
| High resolution limit [Å] | 2.302 | 2.302 |
| Rmerge | 0.021 | 0.466 |
| Number of reflections | 12201 | |
| <I/σ(I)> | 15.5 | 4.93 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 9.4 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1M HEPES-NaOH, 0.2M MgCl2, 30%(v/v) iso-propanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
